Quantitative analysis of proteins by displacement electrophoresis

Quantitative analysis of serum proteins separated by capillary electrophoresis.

The possibility of open tubular capillary electrophoresis for clinical diagnostic use is examined. Capillary electrophoresis was performed in an untreated 50 microns (i.d.) x 100 cm (65 cm to detector) capillary with detection of absorbance at 200 nm. Conditions for the separation of serum proteins without adsorption to the capillary surface were established. Quantitative analyses of serum samp...

Quantitative electrophoresis of serum proteins on paper.

CONSIDERABLE NUMBER OF STUDIES devoted to the quantitative evaluation of paper-electrophoretic patterns have left doubt as to the value of the method as an accurate quantitative procedure. Some workers feel that paper electrophoresis cannot be expected to equal the reproducibility of the moving-boundary technic by virtue of its relative crudeness as compared with the sensitive nature of refract...

Computerized quantitative analysis of coomassie-blue-stained serum proteins separated by two-dimensional electrophoresis.

Two-dimensional electrophoresis in combination with Coomassie Blue staining was refined for use as a quantitative method. Microcomputer software was developed for use with the IBM AT and compatible computers for analyzing the gels. To test the refined method to determine its usefulness in simultaneous measurements of 28 human serum proteins, we measured each protein relative to a single standar...

Distribution of the serum proteins of Syrian hamster as revealed by Starch gel electrophoresis

Behavior of the Serum proteins in starch-agar-gel electrophoresis